A chemical investigation of the aerial parts ofArtemisia vestita Wall. led to the isolation of 12 known sesquiterpenes, including 2 furan-containing sesquiterpenoids and 10 eudesmane sesquiterpene lactones. Their structures were identified as negunfurol (1), schensianol A (2), artemine (3), erivanin (4), 1,5-diepi-artemin (5), acetylartemin (6), naphtho[1,2-b]furan-2(3H)-one, 6-(acetyloxy) decahydro-9a-hydroxy-3,Sa-dimethyl-9-methylene-(3S,3aS,5aS,6S,9aS, ghS) (7), naphtho[1,2-b]furan-2(3H)-one, 6-(acetyloxy)- 3a,4,5,5a,6,7,8,9b-octahydro-8-hydroxy-3,Sa,9-trimethyl- (3S,3aS, SaR,6S, SS,9bS) (8), isoerivanin (9), harrelierin (10), (11S)-1- oxoeudesm-4(14)-eno-13,6a-lactone (11), 1-epi-dehydroisoeranin (12), respectively. All of these compounds were isolated from Artemisia vestita for the first time, and compounds 1 and 2 were isolated from the genus Artemisia for the first time.
A sensitive RP-HPLC-DAD method has been developed and validated for the determination of luteolin and acteoside in the herb ofSiphonostegia chinensis Benth. (Siphonostegiae Herba). Separation was achieved on an Agilent Zorbax SB-Aq C18 column (250 mm×4.6 mm, 5 μm) using a gradient elution with mobile phases of 0.05% phosphoric acid aqueous solution (A) and methanol (B). The assay was carried out at a flow rate of 1.0 mL/min with detection at 310 nm and 350 nm. Luteolin and acteoside showed good linearity in the ranges of 0.0341-0.8172 mg/mL (r2 = 0.9999) and 0.0708-2.832 mg/mL (r2 = 0.9999) with average recoveries of 102.7% and 98.3%, respectively. The contents of luteolin and acteoside varied greatly in 15 samples from different habitats. This is the first report on the quantitative determination of acteoside in Siphonostegiae Herba.