A chemical investigation of the aerial parts ofArtemisia vestita Wall. led to the isolation of 12 known sesquiterpenes, including 2 furan-containing sesquiterpenoids and 10 eudesmane sesquiterpene lactones. Their structures were identified as negunfurol （1）, schensianol A （2）, artemine （3）, erivanin （4）, 1,5-diepi-artemin （5）, acetylartemin （6）, naphtho[1,2-b]furan-2（3H）-one, 6-（acetyloxy） decahydro-9a-hydroxy-3,Sa-dimethyl-9-methylene-（3S,3aS,5aS,6S,9aS, ghS） （7）, naphtho[1,2-b]furan-2（3H）-one, 6-（acetyloxy）- 3a,4,5,5a,6,7,8,9b-octahydro-8-hydroxy-3,Sa,9-trimethyl- （3S,3aS, SaR,6S, SS,9bS） （8）, isoerivanin （9）, harrelierin （10）, （11S）-1- oxoeudesm-4（14）-eno-13,6a-lactone （11）, 1-epi-dehydroisoeranin （12）, respectively. All of these compounds were isolated from Artemisia vestita for the first time, and compounds 1 and 2 were isolated from the genus Artemisia for the first time.
A sensitive RP-HPLC-DAD method has been developed and validated for the determination of luteolin and acteoside in the herb ofSiphonostegia chinensis Benth. （Siphonostegiae Herba）. Separation was achieved on an Agilent Zorbax SB-Aq C18 column （250 mm×4.6 mm, 5 μm） using a gradient elution with mobile phases of 0.05% phosphoric acid aqueous solution （A） and methanol （B）. The assay was carried out at a flow rate of 1.0 mL/min with detection at 310 nm and 350 nm. Luteolin and acteoside showed good linearity in the ranges of 0.0341-0.8172 mg/mL （r2 = 0.9999） and 0.0708-2.832 mg/mL （r2 = 0.9999） with average recoveries of 102.7% and 98.3%, respectively. The contents of luteolin and acteoside varied greatly in 15 samples from different habitats. This is the first report on the quantitative determination of acteoside in Siphonostegiae Herba.
